The GUL-1 Protein Binds Multiple RNAs Involved in Cell Wall Remodeling and Affects the MAK-1 Pathway in Neurospora crassa

The Neurospora crassa GUL-1 is part of the COT-1 pathway, which plays key roles in regulating polar hyphal growth and cell wall remodeling. We show that a bona fide RNA-binding protein (RBP) can associate with 828 “core” mRNA species. When integrity (CWI) challenged, expression over 25% genomic RNA species are modulated (2,628 mRNAs, including mRNA). binds mRNAs genes related to translation, remodeling, circadian clock, endoplasmic reticulum (ER), as well CWI MAPK pathway components. interacts 100 different proteins, stress-granule P-body ER components MAPK, COT-1, STRIPAK complexes. Several additional RBPs were also shown physically interact GUL-1. Under stress conditions, localize affect pathway—evident via altered phosphorylation levels MAK-1, interaction mak-1 transcript, involvement level transcription factor adv-1 . conclude functions multiple cellular processes, regulation mechanism associated MAK-1 stress-response.